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surescripttm firststrand cdna synthesis kit  (Genecopoeia)


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    Structured Review

    Genecopoeia surescripttm firststrand cdna synthesis kit
    Surescripttm Firststrand Cdna Synthesis Kit, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 96/100, based on 270 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/surescripttm+firststrand+cdna+synthesis+kit/SureScript+First-Strand+cDNA+Synthesis+Kit/10__54817_slash_ic__v67n1a06-114-17-25
    Average 96 stars, based on 270 article reviews
    surescripttm firststrand cdna synthesis kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Downregulation of KHSRP enhances carboplatin sensitivity in non-small cell lung cancer.
    Article Snippet: .. After incubation for 6 h at 37 °C, the medium was replaced with fresh complete F-12 K medium and the cells were then exposed to 20 μg/ mL carboplatin for 72 h. Quantitative real-time polymerase chain reaction (qRTPCR) Redzol reagent (SBS Genetech), SureScriptTM FirstStrand cDNA Synthesis Kit (GeneCopeia) and 2×SYBR Green qPCR Master Mix (Servicebio) were used for RNA extraction, reverse transcription, and qPCR amplification in accordance with the manufacturer’s instructions, respectively. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Downregulation of KHSRP enhances carboplatin sensitivity in non-small cell lung cancer.
    Article Snippet: .. After incubation for 6 h at 37 °C, the medium was replaced with fresh complete F-12 K medium and the cells were then exposed to 20 μg/ mL carboplatin for 72 h. Quantitative real-time polymerase chain reaction (qRTPCR) Redzol reagent (SBS Genetech), SureScriptTM FirstStrand cDNA Synthesis Kit (GeneCopeia) and 2×SYBR Green qPCR Master Mix (Servicebio) were used for RNA extraction, reverse transcription, and qPCR amplification in accordance with the manufacturer’s instructions, respectively. ..

    Article Title: Integrating bulk, single-cell, and spatial transcriptomics to identify and functionally validate novel targets to enhance immunotherapy in NSCLC.
    Article Snippet: Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA), and its concentration was determined with a NanoDrop1000 spectrophotometer (NanoDrop, Madison, WI, USA). .. One μg of RNA was reverse-transcribed into cDNA using the SureScriptTM FirstStrand cDNA Synthesis Kit (Genecopoeia, Guangzhou, China). qPCR was conducted in a 20 μL reaction volume on a qTOWER 3 Real-Time PCR Thermal Cycler (Analytik, Jena, Germany) containing 10 μL of 2× BeyoFastTM SYBRGreenqPCRMix (Beyotime, Shanghai, China), 2 μLof primers, 2 μL of cDNA templates, and 6 μL of ddH2O. ..

    Article Title: Expression and functional role of LncRNA GSEC in oral squamous cell carcinoma
    Article Snippet: Vol.11, No.4, Dec 2025 qRT-PCR RNA was purified from samples employing the TriQuick Reagent from Solarbio, Beijing. .. The synthesis of complementary DNA (cDNA) was accomplished utilizing the SureScriptTM FirstStrand cDNA Synthesis Kit obtained from GeneCopeia in the USA. qRT-PCR was performed utilizing a 2×SYBR Green qPCR GSEC-Fwd: 5' AGCAGGCTTGGGATGGTGT 3' GSEC-Rev: 5' GGTTTAGGTGAGCAGGGTGG 3' BCL2-F 5' AGGATTGTGGCCTTCTTTGA 3' BCL2-R 5' GCACCTACCCAGCCTCCGTTAT 3' Bax-F 5' CGCTGACGGCAACTTCAACTG 3' Bax-R 5' ATGAGCACTCCCGCCACAAA 3' CASPASE9-F 5' CGAACTAACAGGCAAGCAGC 3' CASPASE9-R 5' ACATCACCAAATCCTCCAGAAC 3' Ki67-F 5' AAGAAGAGGTCCTACCAGTCG 3' Ki67-R 5' ATCCCAGTTCCATAGTTTGC 3' PCNA-F 5' TCCAGGGCTCCATCCTCAAGA 3' PCNA-R 5' CATATACGTGCAAATTCACCAGA 3' β-actin-F 5' CGTGACATTAAGGAGAAGCTG 3' β-actin-R 5' TAGAAGCATTTGCGGTGGAC 3' Table 2. .. Primer Design Master Mix supplied by Servicebio in Wuhan, employing β-actin as the internal reference gene.

    cDNA Synthesis:

    Article Title: Downregulation of KHSRP enhances carboplatin sensitivity in non-small cell lung cancer.
    Article Snippet: .. After incubation for 6 h at 37 °C, the medium was replaced with fresh complete F-12 K medium and the cells were then exposed to 20 μg/ mL carboplatin for 72 h. Quantitative real-time polymerase chain reaction (qRTPCR) Redzol reagent (SBS Genetech), SureScriptTM FirstStrand cDNA Synthesis Kit (GeneCopeia) and 2×SYBR Green qPCR Master Mix (Servicebio) were used for RNA extraction, reverse transcription, and qPCR amplification in accordance with the manufacturer’s instructions, respectively. ..

    Article Title: Genome-wide identification and expression profiling analysis of the trihelix gene family and response of PgGT1 under abiotic stresses in Platycodon grandiflorus.
    Article Snippet: The trihelix gene family plays an important role in plant growth and abiotic stress responses.. Through the analysis of genomic and transcriptome data, 35 trihelix family members were identified for the first time in Platycodon grandiflorus; they were classified into five subfamilies: GT-1, GT-2, SH4, GTγ, and SIP1.. The gene structure, conserved motifs and evolutionary relationships were analyzed.

    Article Title: Baicalin Inhibits the Infection of CEK Cells by IBV.
    Article Snippet: Baicalin possesses anti-inflammatory, antiviral, and immunomodulatory effects.. However, the antiviral effect of baicalin against infectious bronchitis virus (IBV) has not been well-studied.. In this study, chicken embryo kidney (CEK) cells were used as a model to investigate the antiviral effects of baicalin against the IBV Hebei QX strain.

    Article Title: Integrating bulk, single-cell, and spatial transcriptomics to identify and functionally validate novel targets to enhance immunotherapy in NSCLC.
    Article Snippet: Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA), and its concentration was determined with a NanoDrop1000 spectrophotometer (NanoDrop, Madison, WI, USA). .. One μg of RNA was reverse-transcribed into cDNA using the SureScriptTM FirstStrand cDNA Synthesis Kit (Genecopoeia, Guangzhou, China). qPCR was conducted in a 20 μL reaction volume on a qTOWER 3 Real-Time PCR Thermal Cycler (Analytik, Jena, Germany) containing 10 μL of 2× BeyoFastTM SYBRGreenqPCRMix (Beyotime, Shanghai, China), 2 μLof primers, 2 μL of cDNA templates, and 6 μL of ddH2O. ..

    Article Title: Heme Oxygenase-1 Alleviates Ischemia-Reperfusion Injury by Inhibiting Hepatocyte Pyroptosis after Liver Transplantation in Rats.
    Article Snippet: RT-qPCR was performed as described previously using the SuperReal PreMix Plus (SYBR Green, Roche, Mannheim, Germany) kit and the Mx3005 P Real-Time PCR System (Agilent, Santa Clara, CA, USA) [21]. .. Briefly, total RNA from liver tissues was extracted using TRIzol (Invitrogen, Waltham, MA, USA). cDNA was extracted using SureScriptTM FirstStrand cDNA Synthesis Kit (GeneCopoeia, Guangzhou, China). mRNAs amplification was performed using the following specific primers: HO-1 (102 bp) forward 5′-CCATCCCTTACACACCAGCC-3′, reverse 5′-GGTAGCGGGTATATGCGTGG-3′; β-tubulin (152 bp) forward 5′-GAGGCAGATGGCAGTGACAG-3′, reverse 5′-TGGTTGGGGAACACGGAGTA-3′ (Sangon Biotech, Shanghai, China). ..

    Article Title: Expression and functional role of LncRNA GSEC in oral squamous cell carcinoma
    Article Snippet: Vol.11, No.4, Dec 2025 qRT-PCR RNA was purified from samples employing the TriQuick Reagent from Solarbio, Beijing. .. The synthesis of complementary DNA (cDNA) was accomplished utilizing the SureScriptTM FirstStrand cDNA Synthesis Kit obtained from GeneCopeia in the USA. qRT-PCR was performed utilizing a 2×SYBR Green qPCR GSEC-Fwd: 5' AGCAGGCTTGGGATGGTGT 3' GSEC-Rev: 5' GGTTTAGGTGAGCAGGGTGG 3' BCL2-F 5' AGGATTGTGGCCTTCTTTGA 3' BCL2-R 5' GCACCTACCCAGCCTCCGTTAT 3' Bax-F 5' CGCTGACGGCAACTTCAACTG 3' Bax-R 5' ATGAGCACTCCCGCCACAAA 3' CASPASE9-F 5' CGAACTAACAGGCAAGCAGC 3' CASPASE9-R 5' ACATCACCAAATCCTCCAGAAC 3' Ki67-F 5' AAGAAGAGGTCCTACCAGTCG 3' Ki67-R 5' ATCCCAGTTCCATAGTTTGC 3' PCNA-F 5' TCCAGGGCTCCATCCTCAAGA 3' PCNA-R 5' CATATACGTGCAAATTCACCAGA 3' β-actin-F 5' CGTGACATTAAGGAGAAGCTG 3' β-actin-R 5' TAGAAGCATTTGCGGTGGAC 3' Table 2. .. Primer Design Master Mix supplied by Servicebio in Wuhan, employing β-actin as the internal reference gene.

    Article Title: Withaferin-A induces apoptosis and autophagy in colorectal cancer cell lines via down-regulated expression of histone deacetylase 1.
    Article Snippet: Colorectal cancer (CRC) remains the third most common malignancy worldwide, and there is an urgent need for low-toxicity, mechanism-based preventives or adjuvants.. Withaferin-A (WA), a plant-derived steroidal lactone, exhibits broad antitumor activity; however, its role in CRC and its interactions with epigenetic regulators, such as histone deacetylase 1 (HDAC1), remain unclear.. Therefore, we investigated whether WA suppresses CRC growth by downregulating HDAC1 while inducing apoptosis and autophagy.

    Article Title: Casticin Attenuates Stemness in Cervical Cancer Stem-Like Cells by Regulating Activity and Expression of DNMT1.
    Article Snippet: Human cerv ical cancer (CC) represents the 4th most common gynecological malignancy worldwide, and seriously threatens women's health in developing countries.. It was reported that approximately 570,000 CC cases and 311,000 related deaths occurred in 2018.. The existing therapeutic interventions include surgical resection, radiotherapy, chemotherapy and human papillomavirus (HPV) vaccines, and are effective against CC, but their curative effects are disappointed.

    RNA Extraction:

    Article Title: Downregulation of KHSRP enhances carboplatin sensitivity in non-small cell lung cancer.
    Article Snippet: .. After incubation for 6 h at 37 °C, the medium was replaced with fresh complete F-12 K medium and the cells were then exposed to 20 μg/ mL carboplatin for 72 h. Quantitative real-time polymerase chain reaction (qRTPCR) Redzol reagent (SBS Genetech), SureScriptTM FirstStrand cDNA Synthesis Kit (GeneCopeia) and 2×SYBR Green qPCR Master Mix (Servicebio) were used for RNA extraction, reverse transcription, and qPCR amplification in accordance with the manufacturer’s instructions, respectively. ..

    Reverse Transcription:

    Article Title: Downregulation of KHSRP enhances carboplatin sensitivity in non-small cell lung cancer.
    Article Snippet: .. After incubation for 6 h at 37 °C, the medium was replaced with fresh complete F-12 K medium and the cells were then exposed to 20 μg/ mL carboplatin for 72 h. Quantitative real-time polymerase chain reaction (qRTPCR) Redzol reagent (SBS Genetech), SureScriptTM FirstStrand cDNA Synthesis Kit (GeneCopeia) and 2×SYBR Green qPCR Master Mix (Servicebio) were used for RNA extraction, reverse transcription, and qPCR amplification in accordance with the manufacturer’s instructions, respectively. ..

    Article Title: Baicalin Inhibits the Infection of CEK Cells by IBV.
    Article Snippet: Baicalin possesses anti-inflammatory, antiviral, and immunomodulatory effects.. However, the antiviral effect of baicalin against infectious bronchitis virus (IBV) has not been well-studied.. In this study, chicken embryo kidney (CEK) cells were used as a model to investigate the antiviral effects of baicalin against the IBV Hebei QX strain.

    Article Title: Integrating bulk, single-cell, and spatial transcriptomics to identify and functionally validate novel targets to enhance immunotherapy in NSCLC.
    Article Snippet: Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA), and its concentration was determined with a NanoDrop1000 spectrophotometer (NanoDrop, Madison, WI, USA). .. One μg of RNA was reverse-transcribed into cDNA using the SureScriptTM FirstStrand cDNA Synthesis Kit (Genecopoeia, Guangzhou, China). qPCR was conducted in a 20 μL reaction volume on a qTOWER 3 Real-Time PCR Thermal Cycler (Analytik, Jena, Germany) containing 10 μL of 2× BeyoFastTM SYBRGreenqPCRMix (Beyotime, Shanghai, China), 2 μLof primers, 2 μL of cDNA templates, and 6 μL of ddH2O. ..

    Article Title: Withaferin-A induces apoptosis and autophagy in colorectal cancer cell lines via down-regulated expression of histone deacetylase 1.
    Article Snippet: Colorectal cancer (CRC) remains the third most common malignancy worldwide, and there is an urgent need for low-toxicity, mechanism-based preventives or adjuvants.. Withaferin-A (WA), a plant-derived steroidal lactone, exhibits broad antitumor activity; however, its role in CRC and its interactions with epigenetic regulators, such as histone deacetylase 1 (HDAC1), remain unclear.. Therefore, we investigated whether WA suppresses CRC growth by downregulating HDAC1 while inducing apoptosis and autophagy.

    Article Title: Casticin Attenuates Stemness in Cervical Cancer Stem-Like Cells by Regulating Activity and Expression of DNMT1.
    Article Snippet: Human cerv ical cancer (CC) represents the 4th most common gynecological malignancy worldwide, and seriously threatens women's health in developing countries.. It was reported that approximately 570,000 CC cases and 311,000 related deaths occurred in 2018.. The existing therapeutic interventions include surgical resection, radiotherapy, chemotherapy and human papillomavirus (HPV) vaccines, and are effective against CC, but their curative effects are disappointed.

    Amplification:

    Article Title: Downregulation of KHSRP enhances carboplatin sensitivity in non-small cell lung cancer.
    Article Snippet: .. After incubation for 6 h at 37 °C, the medium was replaced with fresh complete F-12 K medium and the cells were then exposed to 20 μg/ mL carboplatin for 72 h. Quantitative real-time polymerase chain reaction (qRTPCR) Redzol reagent (SBS Genetech), SureScriptTM FirstStrand cDNA Synthesis Kit (GeneCopeia) and 2×SYBR Green qPCR Master Mix (Servicebio) were used for RNA extraction, reverse transcription, and qPCR amplification in accordance with the manufacturer’s instructions, respectively. ..

    Article Title: Heme Oxygenase-1 Alleviates Ischemia-Reperfusion Injury by Inhibiting Hepatocyte Pyroptosis after Liver Transplantation in Rats.
    Article Snippet: RT-qPCR was performed as described previously using the SuperReal PreMix Plus (SYBR Green, Roche, Mannheim, Germany) kit and the Mx3005 P Real-Time PCR System (Agilent, Santa Clara, CA, USA) [21]. .. Briefly, total RNA from liver tissues was extracted using TRIzol (Invitrogen, Waltham, MA, USA). cDNA was extracted using SureScriptTM FirstStrand cDNA Synthesis Kit (GeneCopoeia, Guangzhou, China). mRNAs amplification was performed using the following specific primers: HO-1 (102 bp) forward 5′-CCATCCCTTACACACCAGCC-3′, reverse 5′-GGTAGCGGGTATATGCGTGG-3′; β-tubulin (152 bp) forward 5′-GAGGCAGATGGCAGTGACAG-3′, reverse 5′-TGGTTGGGGAACACGGAGTA-3′ (Sangon Biotech, Shanghai, China). ..

    Agarose Gel Electrophoresis:

    Article Title: Genome-wide identification and expression profiling analysis of the trihelix gene family and response of PgGT1 under abiotic stresses in Platycodon grandiflorus.
    Article Snippet: The trihelix gene family plays an important role in plant growth and abiotic stress responses.. Through the analysis of genomic and transcriptome data, 35 trihelix family members were identified for the first time in Platycodon grandiflorus; they were classified into five subfamilies: GT-1, GT-2, SH4, GTγ, and SIP1.. The gene structure, conserved motifs and evolutionary relationships were analyzed.

    Synthesized:

    Article Title: Genome-wide identification and expression profiling analysis of the trihelix gene family and response of PgGT1 under abiotic stresses in Platycodon grandiflorus.
    Article Snippet: The trihelix gene family plays an important role in plant growth and abiotic stress responses.. Through the analysis of genomic and transcriptome data, 35 trihelix family members were identified for the first time in Platycodon grandiflorus; they were classified into five subfamilies: GT-1, GT-2, SH4, GTγ, and SIP1.. The gene structure, conserved motifs and evolutionary relationships were analyzed.

    Virus:

    Article Title: Baicalin Inhibits the Infection of CEK Cells by IBV.
    Article Snippet: Baicalin possesses anti-inflammatory, antiviral, and immunomodulatory effects.. However, the antiviral effect of baicalin against infectious bronchitis virus (IBV) has not been well-studied.. In this study, chicken embryo kidney (CEK) cells were used as a model to investigate the antiviral effects of baicalin against the IBV Hebei QX strain.

    Infection:

    Article Title: Baicalin Inhibits the Infection of CEK Cells by IBV.
    Article Snippet: Baicalin possesses anti-inflammatory, antiviral, and immunomodulatory effects.. However, the antiviral effect of baicalin against infectious bronchitis virus (IBV) has not been well-studied.. In this study, chicken embryo kidney (CEK) cells were used as a model to investigate the antiviral effects of baicalin against the IBV Hebei QX strain.

    Quantitative RT-PCR:

    Article Title: Baicalin Inhibits the Infection of CEK Cells by IBV.
    Article Snippet: Baicalin possesses anti-inflammatory, antiviral, and immunomodulatory effects.. However, the antiviral effect of baicalin against infectious bronchitis virus (IBV) has not been well-studied.. In this study, chicken embryo kidney (CEK) cells were used as a model to investigate the antiviral effects of baicalin against the IBV Hebei QX strain.

    Article Title: Expression and functional role of LncRNA GSEC in oral squamous cell carcinoma
    Article Snippet: Vol.11, No.4, Dec 2025 qRT-PCR RNA was purified from samples employing the TriQuick Reagent from Solarbio, Beijing. .. The synthesis of complementary DNA (cDNA) was accomplished utilizing the SureScriptTM FirstStrand cDNA Synthesis Kit obtained from GeneCopeia in the USA. qRT-PCR was performed utilizing a 2×SYBR Green qPCR GSEC-Fwd: 5' AGCAGGCTTGGGATGGTGT 3' GSEC-Rev: 5' GGTTTAGGTGAGCAGGGTGG 3' BCL2-F 5' AGGATTGTGGCCTTCTTTGA 3' BCL2-R 5' GCACCTACCCAGCCTCCGTTAT 3' Bax-F 5' CGCTGACGGCAACTTCAACTG 3' Bax-R 5' ATGAGCACTCCCGCCACAAA 3' CASPASE9-F 5' CGAACTAACAGGCAAGCAGC 3' CASPASE9-R 5' ACATCACCAAATCCTCCAGAAC 3' Ki67-F 5' AAGAAGAGGTCCTACCAGTCG 3' Ki67-R 5' ATCCCAGTTCCATAGTTTGC 3' PCNA-F 5' TCCAGGGCTCCATCCTCAAGA 3' PCNA-R 5' CATATACGTGCAAATTCACCAGA 3' β-actin-F 5' CGTGACATTAAGGAGAAGCTG 3' β-actin-R 5' TAGAAGCATTTGCGGTGGAC 3' Table 2. .. Primer Design Master Mix supplied by Servicebio in Wuhan, employing β-actin as the internal reference gene.



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    Genecopoeia surescripttm firststrand cdna synthesis kit
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    https://www.bioz.com/product/surescripttm+firststrand+cdna+synthesis+kit/SureScript+First-Strand+cDNA+Synthesis+Kit/10__54817_slash_ic__v67n1a06-114-17-25
    Average 96 stars, based on 1 article reviews
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